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CytoSMART Multi Lux3 FL

Long-term fluorescence
live-cell imaging
for comparative studies

  • CytoSMART Multi Lux3 FL

Fluorescence live-cell imaging has become an indispensable tool for analyzing the quality and viability of cell cultures. The CytoSMART Multi Lux3 FL is a dual-fluorescence live-cell imaging system, designed for long-term comparative studies and large research teams. The system consists of four compact Lux3 FL fluorescence microscopes, equipped with two fluorescent (green and red) and one brightfield channel that allow to analyze multiple cell features simultaneously. It fits comfortably in any standard incubator, still allowing to accommodate cell culture plates and flasks. The CytoSMART Multi Lux3 FL imaging system is a cost-effective solution for large research groups and educational facilities that aim to increase their throughput and efficiency.

  • Flexible – connect devices to the same laptop and control them individually
  • Incubator-friendly – study cells in their desired culture environment
  • Designed for large research groups and educational facilities – increase the efficiency of your lab group
  • Indispensable tool for long-term, large-scale comparative studies – run up to four individual experiments simultaneously for days or weeks

Large-scale comparative studies

Compare cell cultures using automated image analysis

With the CytoSMART Multi Lux3 FL system, it is possible to monitor up to four cell cultures subjected to different culture conditions (e.g. growth medium, pH) and treatments, as well as positive and negative controls. The cells can be monitored directly from inside a standard CO2-incubator, minimizing the unnecessary cell handling and disruption. The images and time-lapse movies are then automatically processed using the CytoSMART image analysis software. This results in increased throughput and produces more statistically accurate results. The Multi Lux3 FL system can be used for a variety of applications, including analysis of cell viability and cellular processes, single-cell migration, and co-culture models.

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Solution for large research groups

Being a part of a large research group can be associated with a limited access to laboratory equipment and resources. The CytoSMART Multi Lux3 FL system consists of four fluorescence microscopes, allowing to run up to four individual imaging experiments simultaneously. Users can start and stop their experiments at any time, without affecting the time-lapse imaging experiments of other lab members. The CytoSMART Cloud offers remote access to experimental data and automated image analysis, ensuring effective data organization and storage for all research group members.

Technical specifications

Number of Lux3 FL units 4
Channels Brightfield, green, and red fluorescent channels
Peak wavelength / bandwidth Green – excitation: 452/45 nm; emission: 512/23 nm
Red – excitation: 561/14 nm; emission: 630/90 nm
Magnification 10× (digital zoom 20×)
Image resolution 2072 × 2072 pixels
Field of view (per device) 1.45 mm × 1.45 mm
Camera 6.4 MP CMOS
Light source LED
Data formats JPG, TIFF, MP4, XLSX
Data storage Unlimited cloud storage & unlimited number of user accounts
Computer requirements Windows 10, USB 3.0 (× 4)
Culture vessels Any transparent culture vessel: well-plates, petri dishes, flasks, microfluidic chips, and custom culture vessels
Dimensions (L × W × H; per device) 16.6 cm × 14 cm × 13.5 cm
Weight (per device) 1.3 kg
Operating conditions 5 – 40 °C, 20 – 95% humidity
Warranty 1-year parts and labor
Extra features Template functionality
Object count
Research use only. Not intended for diagnostic purposes

Frequently Asked Questions

The CytoSMART Multi Lux3 FL is a live-cell imaging system that employs four compact fluorescence microscopes, designed to be placed inside an incubator to monitor cell cultures. The CytoSMART Cloud environment provides real-time updates on the status of running experiments and allows to access images and time-lapse videos remotely.

If you already have a single CytoSMART Lux3 FL device in your lab, upgrading to the Multi Lux3 FL Kit can be done by simply ordering extra CytoSMART Lux3 FL devices. All devices can operate via a single laptop (or desktop), as long as the laptop is equipped with a multi-port USB 3.0 adapter.

The CytoSMART Lux3 FL Duo Kit is compatible with a wide range of different culture vessels, including T-flasks (T-25 to T-250), multi-well plates (6 – 384-well plates), microfluidic chips, flat tubes, petri dishes, and slides.

The remote functionality of the CytoSMART Multi Lux3 FL runs on cloud-based computing. In this cloud environment, the images and videos are stored and can be accessed using user-specific login details. Next to unlimited data storage and unlimited number of user accounts, automated image analysis can be performed in the CytoSMART Cloud portal.

The devices are easy to clean using lint-free wipes and ethanol (70%) or isopropyl alcohol (IPA). Do not use acetone to clean the devices. Please be aware that the devices cannot be autoclaved.

After sterilizing with ethanol or IPA, the devices can be used in a clean room.

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