CytoSMART Omni

CytoSMART Omni

Live-cell imaging
for high-throughput

  • CytoSMART Omni

CytoSMART Omni

Being able to monitor cell cultures over time provides a great insight into their physiology and function. Live-cell imaging microscopes open up novel and exciting avenues to study cellular health, viability, colony formation, migration, and cellular responses to external stimuli.

To help life science researchers improve their understanding of cellular processes, CytoSMART Technologies has developed an automated bright-field microscope that can visualize an entire surface of a cell culture vessel and operate from inside a standard CO2-incubator, biological safety cabinet or on a benchtop. Not limited to a specific type or quantity of culture vessels, the CytoSMART Omni captures cellular behavior by creating high-quality time-lapse videos for days or even weeks at a time.

效果很好,我向所有从事细胞培养工作的人推荐 CytoSMART Omni 设备

Hanan Osman-Ponchet PKDERM

"每个实验室都有CytoSMART Omni 设备。它操作非常容易。该软件非常使用者友好和直观。您可以获得在线图像,并且可以随时随地关注您的细胞。在 PKDERM,我们使用 Omni 设备进行伤口愈合测试,使用Ibidi培养插件进行划痕测试。我们取得了非常好的结果,我们的合作伙伴和客户都非常满意,尤其是当您可以观看有关伤口如何恢复的视频时。"

Application Area: 伤口愈合、皮肤病学、细胞成像、筛查、动态评估

Easy to handle, great results!

Thomas Dechat Ludwig Boltzmann Institute of Osteology

"The Omni allows to image cells over several days up to weeks and so learn a lot of new facts about their behavior. The microscope can be placed in an incubator and the medium can be changed in-between, without impairing imaging. Therefore no effects concerning the change of environmental conditions are observed. This is very important for our research on osteoblast differentiation, a process which can take up to five weeks. The microscope is very easy to handle and to control from outside the incubator. And you can always count on the people from CytoSMART for technical support."

Application Area: 活细胞成像

Top-end technology that keeps on improving.

Manuel Reitberger German Cancer Research Center (DKFZ)

"Constant development of analysis software in cloud is excellent. Hardware improvements are immediately implemented into the device. Great support by the company."

Application Area: 癌症研究

Amazing for long-term imaging of whole wells and plates!

Neshika Wijewardhane King's College London

"Very easy product to use. Does require a bit of setup, but the company sends out a representative to help with this. Also, they ask what I want to use the device for and really tailor the experience. Love that it can be used inside an incubator as I had trouble with long-term imaging with on stage incubators for big microscopes. Also, don't need to worry about fluctuations in temperature and oxygen percentages and deal with oxygen tanks. The confluency data is very useful and produces graphs ready for you to use. Maybe have more options for well/plates to be able to scan."

Application Area: 长期实时成像

Very good and recommended.

Ebrahim Hamza Freie Universität Berlin

"The device was very easy to set up, run experiments in and the data it produces is reliable."

Application Area: 监测细胞培养物的增殖

Useful tool that allows for fast progression.

Johannes Vandaele KU Leuven

"Compact and nicely-designed piece of equipment. Very straightforward to use in terms of hardware and software."

Application Area: 监测细胞生长


Martin Sprick 海德堡干细胞技术与实验医学研究所

"CytoSMART Omni 非常适合进行动力学分析以绘制细胞增殖图,我们将其用作了解胰腺癌细胞系耐药性的工具"

Application Area: 细胞增殖

Ensure sample stability while imaging

Produce bright-field images without moving your samples

Acquiring images can be highly disturbing to living cells. Environmental shock, like sudden changes in the atmosphere and mechanical disturbance can negatively influence cell viability. The CytoSMART Omni rapidly captures bright field images at cell-level detail without disturbing the cells. Users can set the desired imaging parameters using their own computer and the CytoSMART Omni will follow that scanning regime. There is no need to move a culture vessel on the sample stage, since the camera moves along the platform to capture the entire area of the vessel. Automated time-lapse imaging eliminates the need for any manual interference. Imaging using such a stable system prevents unwanted disturbances and reduces imaging artifacts.

Complete overview of sample confluency

Achieved by automated full plate scanning and image stitching

Manual handling and cell seeding can cause a variable density distribution within culture vessels. Randomly selecting several areas of interest or tile scanning is common practice to overcome that issue, however this is either time-consuming to set-up or to post-process. The CytoSMART Omni automatically scans complete well surface areas and instantly stitches these images to give users a complete overview of cell coverage.

Continuous imaging provides real-time insight into cellular processes

Perform kinetic assays over the course of days or even weeks

Time-lapse imaging allows you to pinpoint important events in the progression of the cell culture from experiment to experiment. Uncover attachment and detachment rates, evaluate events like cell death and compare growth rates. Data collection using video monitoring allows users to capture changes within samples and compare the rate of change between samples. With the integrated Continue Experiment feature, it is now possible to perform multiple transient screens in parallel to an ongoing long-term kinetic assay by simply pausing the running experiment.

Compatibility with any transparent culture vessel

Imaging of full plates, flasks, dishes and microfluidic devices

Cell based assays and subculturing all benefit from standardization. Choosing the right vessel and going through the process of understanding how cells behave in said vessel is vital for reproducibility. The CytoSMART Omni is highly flexible and is compatible to any transparent vessel that is lower than 55 mm. All vessels with a surface area smaller than 99*131 mm can be imaged in full (6 – 384-well plates, T-25 – T225, Petri dishes, triple flasks, and HYPERFlasks).

Efficient screening of culture multiwell plates

The CytoSMART Omni can scan up to 6 vessels per hour

Omni plate stacking feature

High-throughput experiments are not limited to a single culture vessel. The CytoSMART Omni can process multiple plates in quick succession, automatically stitching acquired images and safely storing data for each individual plate.

The CytoSMART Omni is ideal to perform kinetic assays to map cell proliferation, which we use as a tool to understand drug resistance in pancreatic cancer cell lines.

Dr. Martin Sprick | Senior Group Leader

Heidelberg Institute for Stem Cell Technology and Experimental Medicine

The CytoSMART  Omni is ideal to perform kinetic assays to map cell proliferation, which we use as a tool to understand drug resistance in pancreatic cancer cell lines.
  • Incubator-friendly
  • Label-free cellular assays
  • Time-lapse videos
  • Image stitching
  • Cloud-based image analysis algorithms

Would you like to test if the CytoSMART Omni is beneficial for your laboratory?

Confluence omni bf
Kinetic assays: cell proliferation, migration,
colony formation & more

Image analysis can be used to extract valuable information from time-lapse videos. Use our cell monitoring software to investigate and compare parameters like cell confluence, area infiltration (wound healing assays) and growth rate.

Perform analysis at desired culturing environment

The CytoSMART Omni is designed for your incubator

Live-cell imaging systems require environmental control during the experiment. To ensure the proper environmental conditions, users can place the compact CytoSMART Omni inside any standard cell culture incubator. The smooth curvatures of the CytoSMART Omni platform ensures minimal airflow disturbance. This ensures that your samples stay in their desired, homogeneous environment throughout the entire experiment. What's more, the versatile nature of the CytoSMART Omni allows it to be used directly at a bench or biological safety cabinet, ensuring the most optimal setting for conducting an imaging experiment.

Technical specifications

Dimensions (L × W × H) 396 mm × 345 mm × 171 mm
Weight 9 kg
Optics Brightfield with digital phase contrast
Magnification 10× fixed objective
Light source LED
Camera 5.0 MP CMOS
Scan area 99 mm × 131 mm
Exported formats JPG, XLSX & MP4
Well plate types 6 – 384 well plates
Culture flask types Petri dishes, T25 – T225, triple flasks, and HYPERFlasks
Other labware Anything transparent and lower than 55 mm
Operating environment 5 – 40 °C, 20 – 95% humidity
Support Via email and live chat
Research use only. Not intended for diagnostic purposes



Brochure CytoSMART Omni

Omni-brochure-v3-148x148mm-sprea ( PDF - 6 MB )

Case Study Fibroblast Scratch Assay Protocol Omni

Case-Study-Fibroblast-Scratch-Assay-Protocol-Omni ( PDF - 771 KB )

AP Pnote Omni Collective Cell Migration

APPnote-Omni-Collective-Cell-Migration ( PDF - 2 MB )

AP Pnote Cyto SMART Promo Cell Kinetic Analysis of in vitro wound closure by keratinocytes

APPnote-CytoSMART_PromoCell-Kinetic-Analysis-of-in-vitro-wound-closure-by-keratinocytes ( PDF - 2 MB )

Cell culture optimization - appnote

Using-Bright-Field-Video-Monitoring-Inside-Incubator-For-Cell-Culture-Optimization_200228_143933 ( PDF - 1 MB )

Cyto SMART Omni Whitepaper Metabolic Activity Assay vs Confluence

CytoSMART-Omni-Whitepaper-Metabolic-Activity-Assay-vs-Confluence ( PDF - 353 KB )

AP Pnote The effect of Blebbistatin and Cytochalasin D

APPnote-The-effect-of-Blebbistatin-and-Cytochalasin-D ( PDF - 628 KB )

Cell viability comparing metabolism assays to kinetic bright field microscopy analysis

Cell-viability-comparing-metabolism-assays-to-kinetic-bright-field-microscopy-analysis ( PDF - 2 MB )

Cytotoxicity assay evaluating drug toxicity using label free microscopy

Cytotoxicity-assay-evaluating-drug-toxicity-using-label-free-microscopy ( PDF - 2 MB )

Live Cell Imaging and Quantitative Analysis of Wound Healing Assays

Live-Cell-Imaging-and-Quantitative-Analysis-of-Wound-Healing-Assays ( PDF - 2 MB )

HYPER Flask cell culture monitoring using the Cyto SMART Omni

HYPERFlask-cell-culture-monitoring-using-the-CytoSMART-Omni ( PDF - 2 MB )

Cyto SMART Omni label free assays colony formation

CytoSMART_Omni-label-free_assays-colony-formation ( PDF - 2 MB )

Cyto SMART Omni wound healing assay

CytoSMART_Omni-wound_healing_assay ( PDF - 1 MB )

App Note Development of a high throughput 3 D in vitro wound healing model 2021 11 22 125143 vxgn

AppNote-Development-of-a-high-throughput-3D-in-vitro-wound-healing-model_2021-11-22-125143_vxgn ( PDF - 748 KB )

AP Pnote1003 Omni 2022 02 08 093111 uyhi

APPnote1003-Omni_2022-02-08-093111_uyhi ( PDF - 639 KB )

AP Pnote1001 Omni 2022 02 08 094017 yjno

APPnote1001-Omni_2022-02-08-094017_yjno ( PDF - 1 MB )

Frequently Asked Questions

The CytoSMART Omni is an inverted brightfield microscope that uses digital phase contrast imaging to visualize live cells without the addition of fluorescent or colorimetric dyes. Samples are illuminated using a LED and recorded with a moving camera that is positioned below the sample stage. The camera scans the sample stage and acquires a series of sequential images. One complete scan takes approximately 6 minutes, generating around 6500 snapshots. These are stitched to form an image with a surface area of 99 mm × 131 mm. The images are then uploaded to the CytoSMART™ Cloud, where they can be analyzed using our image analysis algorithms.

You can specify the interval rate between 1 - 24 hours or choose to perform a single scan.

The magnification is equivalent to a 10x objective of a standard brightfield microscope.

Confluence, scratch analysis, and colony detection are currently a part of the image analysis software package. Users always have the option to download the raw image data and perform their own analysis.

Yes, the CytoSMART Omni is designed to be used inside a cell culture incubator. Its hardware and electronics can operate at 5 - 40 °C and between 20 - 95% humidity.

No, our image analysis algorithms are optimized to be used in label-free assays, so you don’t have to add any dyes to your culture, providing a non-invasive analysis of your cells.

Yes, the device can only be used with a desktop or laptop running on Windows 10 or above, with a USB 3.0 port (which can also be purchased at CytoSMART™). A WiFi or wired Ethernet connection is necessary to be able to connect to the CytoSMART™ Cloud for data storage and analysis.

Any culture vessel that is lower than 55 mm (height of the light arc) can be scanned. Some examples include 6 - 384-well plates, petri dishes, T25 - T225, triple flasks, and HYPERFlasks. However, the size of the scan area is limited to 99 mm × 131 mm, which fits a complete T175 flask.

Yes, after sterilizing with ethanol (70%) or isopropyl alcohol (IPA), the device can be used in a cleanroom. Do not use acetone to clean the device, also the device cannot be autoclaved.

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